Aim of our study is investigation of the genetic relationships between Salmonella spp isolates which taken
from human stool isolates and Salmonella spp isolates which taken from cattle meat. The total number
of study isolates is one hundred (50 cattlebeef and 50human stools. Bacterial isolation and bacterial
identification are used for testing the samples for culturing and examined by biochemical tests then detected
by using 16S rRNA gene in PCR that designed by NCBI and make DNA sequencing for two positive
isolates. Our results revealed that percentage of Salmonellaspp was 8/50(16%) which isolated from cattle
beef and was 10/50(20%) which isolated from human stool. The PCR was demonstrated highly specific
and sensitive. DNA sequencing of part of 16S rRNA was show highly similarity with NCBI-Blast data and
the phylogenetic study was reveal genetic homology at (0.5 genetic change) between animals and human
isolates and then the gene sequence deposited into NCBI-Genbank accession numbers accession numbers
(MN523665.1) and (MG388337.1). In conclusion, our study is done for first-time in Iraq of the genetic
correlating between Salmonellaspp which isolated from cattle meat and human stool. So, it is important to
know the cattle meat is significant source for spreading infection between human and animals. … قراءة المزيد